Journal: Journal of visualized experiments : JoVE

Article Title: Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

doi: 10.3791/57077

Figure Lengend Snippet:

Article Snippet: Name Company Catalog Number Comments Schneider's Drosophila Medium Thermo Fisher Scientific (Gibco) 21720024 1.1.1), 2.1.2) Fetal Bovine Serum GE Healthcare Life Sciences (HyClone) SH30070.03HI 1.1.1), 2.1.2) Filter (0.22 μL) RESTEK 26158 1.1.1) Strainer (100 μm) Greiner bio-one 542000 1.2.1), 2) Stereo microscope Amscope SM-1BSZ-L6W 1.2), 2) Glass capillary Fisher Scientific 21-171-4 1.1), 1.2), 2) Capillary puller Narishige International USA, Inc. PC-10 1.1.3) Mineral oil Snow River Products 1.1.4) Nanoinjector Drummond Scientific Company 3-000-204 1.1), 1.2), 2.2) Forceps VWR 82027-402 1.1.5), 1.2), 2), 3.1.7) CO 2 delivery apparatus Genesee Scientific 59-122BC 1.2), 2) Trypan Blue Thermo Fisher Scientific (Gibco) 15250061 1.3) Hemocytometer Hausser Scientific 3100 1.3) 24 well plate Greiner bio-one 662160 1.4), 2.2) Coxiella burnetii - mCherry Dr. Heinzen, R. 1.4), 2.2) Drosophila fruit juice plates Cold Spring Harbor Protocols 2.1) http://cshprotocols.cshlp.org/content/2007/9/pdb.rec11113.full Agar Fisher Bioreagents BP1423-500 2.1.1.1) Methyl paraben Amresco 0572-500G 2.1.1.2) Absolute ethanol Fisher Bioreagents BP2818-500 2.1.1.2) Welch's 100% Grape juice frozen concentrate, 340 mL Amazon B0025UJVGM 2.1.1.3) Petri dishes, 10 × 35 mm Fisher Scientific 08-757-100A 2.1.1.4) Microscope cover glass Fisher Scientific 12-545-80 1.4.4), 2.2.2) Yeast, Bakers Dried Active MP Biomedicals 0210140001 2.1) Add 2 parts of water to 1 part of yeast (v/v) Tungsten needle Fine Science Tools 10130-20 2.1) Holding forceps VWR HS8313 2.1) Paraformaldehyde Fisher Scientific FLO4042-500 3.1.3) Triton X-100 Fisher Scientific BP151-500 3.1.3) Bovine Serum Albumin Fisher Scientific BP9706-100 3.1.3) 4',6-diamidino-2-phenylindole Thermo Fisher Scientific 62247 3.1.4) Antifade mounting medium Thermo Fisher Scientific P36930 3.1.6) Confocal microsope Leica TCS SP8-X White Light Confocal Laser Scanning Microscope 3.2) 3D imaging reconstruction software Leica LASX with 3D visualization module 3.3) Microscope slides Fisher Scientific 12-552-3 3.1.6) Invertebrate iridescent virus 6 (IIV6) Dr. Teixeria, L. 4) PLoS Biol, 6 (12), 2753-2763, doi: 10.1371/journal.pbio.1000002, (2008) Listeria monocytogenes ATCC strain: 10403S 4) Listeria monocytogenes strain 10403S (Bishop and Hinrichs, 1987) was grown in Difco Brainheart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30 °C.

Techniques: Microscopy, Laser-Scanning Microscopy, Imaging, Software, SYBR Green Assay, Real-time Polymerase Chain Reaction, Western Blot, Produced

Journal: Journal of visualized experiments : JoVE

Article Title: Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

doi: 10.3791/57077

Figure Lengend Snippet:

Article Snippet: Name Company Catalog Number Comments Schneider's Drosophila Medium Thermo Fisher Scientific (Gibco) 21720024 1.1.1), 2.1.2) Fetal Bovine Serum GE Healthcare Life Sciences (HyClone) SH30070.03HI 1.1.1), 2.1.2) Filter (0.22 μL) RESTEK 26158 1.1.1) Strainer (100 μm) Greiner bio-one 542000 1.2.1), 2) Stereo microscope Amscope SM-1BSZ-L6W 1.2), 2) Glass capillary Fisher Scientific 21-171-4 1.1), 1.2), 2) Capillary puller Narishige International USA, Inc. PC-10 1.1.3) Mineral oil Snow River Products 1.1.4) Nanoinjector Drummond Scientific Company 3-000-204 1.1), 1.2), 2.2) Forceps VWR 82027-402 1.1.5), 1.2), 2), 3.1.7) CO 2 delivery apparatus Genesee Scientific 59-122BC 1.2), 2) Trypan Blue Thermo Fisher Scientific (Gibco) 15250061 1.3) Hemocytometer Hausser Scientific 3100 1.3) 24 well plate Greiner bio-one 662160 1.4), 2.2) Coxiella burnetii - mCherry Dr. Heinzen, R. 1.4), 2.2) Drosophila fruit juice plates Cold Spring Harbor Protocols 2.1) http://cshprotocols.cshlp.org/content/2007/9/pdb.rec11113.full Agar Fisher Bioreagents BP1423-500 2.1.1.1) Methyl paraben Amresco 0572-500G 2.1.1.2) Absolute ethanol Fisher Bioreagents BP2818-500 2.1.1.2) Welch's 100% Grape juice frozen concentrate, 340 mL Amazon B0025UJVGM 2.1.1.3) Petri dishes, 10 × 35 mm Fisher Scientific 08-757-100A 2.1.1.4) Microscope cover glass Fisher Scientific 12-545-80 1.4.4), 2.2.2) Yeast, Bakers Dried Active MP Biomedicals 0210140001 2.1) Add 2 parts of water to 1 part of yeast (v/v) Tungsten needle Fine Science Tools 10130-20 2.1) Holding forceps VWR HS8313 2.1) Paraformaldehyde Fisher Scientific FLO4042-500 3.1.3) Triton X-100 Fisher Scientific BP151-500 3.1.3) Bovine Serum Albumin Fisher Scientific BP9706-100 3.1.3) 4',6-diamidino-2-phenylindole Thermo Fisher Scientific 62247 3.1.4) Antifade mounting medium Thermo Fisher Scientific P36930 3.1.6) Confocal microsope Leica TCS SP8-X White Light Confocal Laser Scanning Microscope 3.2) 3D imaging reconstruction software Leica LASX with 3D visualization module 3.3) Microscope slides Fisher Scientific 12-552-3 3.1.6) Invertebrate iridescent virus 6 (IIV6) Dr. Teixeria, L. 4) PLoS Biol, 6 (12), 2753-2763, doi: 10.1371/journal.pbio.1000002, (2008) Listeria monocytogenes ATCC strain: 10403S 4) Listeria monocytogenes strain 10403S (Bishop and Hinrichs, 1987) was grown in Difco Brainheart infusion (BHI) broth (BD Biosciences) containing 50 μg/ml streptomycin at 30 °C.

Techniques: Microscopy, Laser-Scanning Microscopy, Imaging, Software, Virus, cDNA Synthesis, SYBR Green Assay, Real-time Polymerase Chain Reaction, Western Blot, Produced